Dr. Tolu Olupona majored in Biochemistry at the University of California, Davis and graduated with Honors. She was awarded a UC Davis Chancellor Achievement award. She received her medical degree from New York University School of Medicine and completed training in Adult psychiatry at New York University School of Medicine. She completed training in Child and Adolescent Psychiatry at the Columbia and Cornell University New York Presbyterian Hospital. She is board certified in Child, Adolescent and Adult Psychiatry.

After fellowship, she worked as an assistant clinical professor in Psychiatry and Pediatrics at the Mount Sinai School of Medicine Adolescent Health Center where she provided direct clinical care, taught and supervised clinicians. She is currently the program director of Adult Psychiatry Residency Training Program at One Brooklyn Health Interfaith Medical Center.

She has been selected as a Distinguished Fellow of the American Psychiatry Association in May 2020. She received American Psychiatry Association Assembly Resident-Fellow Mentor Award for outstanding mentorship of residents, fellows and medical students in Psychiatry in May 2023. She has served on the advisory board of Nguvuhealth, a mental health startup.

She is a past president of the Brooklyn Psychiatry Society District Branch of the American Psychiatric Association.